Monitoring and Molecular Diagnosis of Interactive Effects of Chemical and Bio Pest Control Agents

The present work was carried out at laboratory of pesticides Biotechnology and Molecular Toxicology, Department of Plant Protection, Faculty of Agriculture, Zagazig.The present investigation will undertake to study the interactive between the entomopathogenic bacteria and chemical pesticides (insecticides, fungicides and herbicides) and the effects on bacteria toxicity, using diagnostic techniques to detect the difference which happen for bacteria toxin and effects on health and environmental potential risk.Our results in vitro suggest that the chemical pesticides inhibited growth of Bacillus thuringiensis at high concentrations. While the obtained results in vivo did not show a synergistic action between B. thuringiensis and both profenofos and atrazine in contrast to metalaxyl-M + copper oxychloride at the concentration of Lc25 + Lc25. The mixture of chemical pesticides with B. thuringiensis was an antagonistic action where reduced total protein content and enzymes glutathione s-transferase and acetylcholinesterase activities, In contrast it increased enzyme alkaline phosphatase activity. The results of polymerase chain reaction- Random Amplification of Polymorphic DNA and sodium dodecyl sulphate - polyacrylamide gel electrophoresis suggested that the chemical pesticides are mutant to B. thuringiensis. The results on rats revealed that most mixtures of chemical and bio pesticides were an antagonistic action, also adding chemical pesticides to B. thuringiensis increased pathologists.