Studies On Certain Hemipterous Insects Infesting Some Fruit Sharkia Governorate Egypt

Rearing of D. maidis and determination the transmission efficient of MBSM by D. maidis and the minimum and maximum incubation and retentionperiods of MBSM in D. maidis. Extraction of phytoplasma DNA from both plant and insect tissues .Analyzing DNAS extracted from the MBSM unfected maize, and D. maidis for presence of the causal phytoplasma by using PCR method. Using the latter RNA primers during a comparative study of several maize bushy stunt phtoplasma isolates originating from different geographic locaities.