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Investigation of mtDNA control region sequences in an Egyptian population sample
Faculty
Veterinary Medicine
Year:
2013
Type of Publication:
Article
Pages:
338-341
Authors:
Elmadawy, Mostafa Ali, Nagai, Atsushi, Gomaa, Ghada M, Hegazy, Hanaa M. R, Shaaban, Fawzy Eid, Bunai, Yasuo
DOI:
10.1016/j.legalmed.2013.06.006
Journal:
LEGAL MEDICINE ELSEVIER IRELAND LTD
Volume:
15
Research Area:
Legal Medicine; Biomedical Social Sciences
ISSN
ISI:000327755400012
Keywords :
Mitochondrial DNA, Hyper variable regions, Forensic data, Egypt delta
Abstract:
The sequences of mitochondrial DNA (mtDNA) control region were investigated in 101 unrelated individuals living in the northern region of Nile delta (Gharbia, N = 55 and Kafrelsheikh, N = 46). DNA was extracted from blood stained filter papers or buccal swabs. HV1, HV2 and HV3 were PCR amplified and sequenced; the resulted sequences were aligned and compared with revised Cambridge sequence (rCRS). The results revealed presence of total 93 different haplotypes, 86 of them are unique and 7 are shared haplotypes, the most common haplotype, was observed with a frequency, 2.97\% of population sample. High mtDNA diversity was observed with genetic diversity and power of discrimination, 0.9982 and 0.9883, respectively. In this dataset the west Eurasian haplogroups predominated over the African haplogroups. The results would be useful for forensic examinations and human genetic studies. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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