The current study aimed to compare between open and tow portals laparoscopic techniques for colopexy in dogs. Twenty healthy adult mongrel male dogs were divided equally into two groups: open colopexy (OC) group (n=10) and laparoscopic colopexy (LC) group (n=10). Open colopexy was performed through laparotomy while laparoscopic colopexy was performed using two port technique and transabdominal fixation of the colon. WBCs, serum CRP, ultrasongraphic, laparoscopic second look and histopathological examinations were conducted. Results declared significant increase in leukocytic counts and serum CRP in OC as compared to LC group. Moreover, ultrasonography showed intensive hyperechogenisty between the descending colon and the left abdominal wall. Laparoscopic second look revealed the adhesion between the descending colon and the left abdominal wall in both groups and the omental adhesions with the spleen and the left abdominal wall in OC group. Histopathological examination revealed that granulation tissue, inflammatory cells and tissue degeneration in OC technique were more pronounced than that in LC technique. In conclusion, LC is more recommendable than OC for its non-invasive technique, offered the advantages of direct visualization of the abdominal cavity and reduced incidence of complications.