Testicular torsion is a common disorder in males and results in blockage of testicular circulation with subsequent damage of testicular germ cells. The current work aimed to compare the therapeutic efect of platelet‑rich plasma (PRP) and injectable platelet‑rich fbrin (i‑PRF) on torsion/detorsion (T/D) injury in rats. Forty mature male Wister rats were arranged into 4 groups; (1) Control, (2) T/D, (3) T/D + PRP, and (4) T/D+ i‑PRF. The right testis was twisting 1080° clockwise for 3 h in groups 2, 3 and 4, then 10 μl of PRP or i‑PRF was injected intra‑testicular 3 h after detorsion in groups 3 and 4, respectively. After 30 days postoperatively, the semen quality and hormonal assay were improved in PRP and i‑PRF‑treated groups with superiority of i‑PRF (P < 0.001). High signifcance of Catalase, Glutathione Peroxidase (GPx), Superoxide Dismutase, Interleukin‑1β (IL‑1β), Caspase‑3 andTumor necrosis factor‑α (TNF‑α) was reported in treated rats with PRP and i‑PRF (P < 0.001) with superiority to i‑PRF‑treated rats P < 0.001). Testicular histoarchitectures were improved in PRP and i‑PRF‑treated rats with superiority of i‑PRF‑treated rats. It was concluded that PRP and i‑PRF have regenerative efcacy on testicular damage after induced T/D injury with a superior efcacy of i‑PRF.