This study is an attempt to search for a fungal control agent for the desert locust, Schistocerca gregaria (Forskål), based on naturally associated fungi with such insect pest and originally isolated from it. A colony of S. gregaria was reared under laboratory conditions. The naturally infected nymphs, adults and eggs were collected and checked for entomopathogenic fungi. A total of 33 fungal species, belong to 12 genera were isolated. The highest total count of isolated fungi was that from the 5th instar nymphs (33.8 colony forming unit, CFU per nymph), and the lowest count was isolated from egg stage (7.39 CFU/egg). Among the fungal species, Penicillium stoloniferum showed the highest frequency of occurrence from the adult stage, Aspergillus ochraceus and Mucor circinelloides from the nymphal stage and, Fusarium lateritium from the egg stage. After preliminary tests, 14 fungal species were selected to determine their lethal against the 1st nymphal instar. Results showed that the Penicllium aurantiogriseum spores, caused the rapid lethal effect, with LT50 of 4 days after infection.