This paper examined alcoholic extract of Cordia myxa fruits (CMF) and identified the existence of bioactive compounds, including alkaloids, saponins, flavonoids, and steroids. CMF was found to have good antioxidants, antibacterial and antifungal effects. Another purpose of the research was to examine its efficacy as a natural meat preservative and also to learn the ideal concentration in order to achieve antimicrobial activity. Beef samples that were treated with 1, 3 and 5% CMF extract and kept in refrigerator at 4 C temperature acquired evident antimicrobial effects on Staphylococcus aureus, Salmonella Enteritidis, Pseudomonas aeruginosa and Escherichia coli. The concentration of 5 percent produced the strongest decrease in the growth of bacteria. This paper incorporated the concept of molecular docking modeling to analyze the highly competent HPLC analysis and the element of effectiveness in inhibition of keyanti bacterial (3HWW, 1D0V, 2RKZ, 2Q0I). P-coumaric acid exhibited huge binding affinities to all target proteins. These outcomes demonstrated that these compounds have appropriate physicochemical characteristics and high biological availability, in line with the Lipinski Rule of Five.The presented results imply that these compounds can be used as the potential antimicrobial agents and food preservatives, provided that their structure is optimized. As such, Cordia myxa extract is a potential ingredient that can be used to preserve food as well as increase shelf life of food products in a natural and safe manner.