Obstructive urolithiasis is a common surgical disorder affecting young ruminants, especially in winter season. The aim of the present study was to evaluate the efficacy of dorsal urethrotomy technique in relieving urolithiasis in buffalo calves with ruptured urinary bladder. Ten non-castrated male buffalo calves, 4-7 months old, were included in the study. The animals had a history of anuria and diagnosed as obstructive urolithiasis with bladder rupture based on clinical, ultrasonographic and biochemical examinations. Dorsal urethrotomy technique was performed in the affected calves and a Ryle’s tube was applied in the urethra for four days postoperatively. The animals were followed for three months after surgery to record complications and recurrence of obstruction. Serum levels of creatinine (7.6±1.67) and blood urea nitrogen (BUN) (54.2±12.65) were significantly higher than the clinically healthy animals (1.4±0.18 and 21.1±6.05, respectively). Nine out of ten calves recovered successively without complications or recurrence of obstruction for three months after surgery. Only one case had re-obstruction 20 days post-surgical interference. In conclusion, dorsal urethrotomy technique is a successful surgical procedure in relieving urolithiasis in calves with ruptured bladder with minimal postoperative complications and re-obstruction.