This study performed a genome-wide identification and characterization of the Dof transcription factor gene family. The analysis revealed the number, structure, chromosomal distribution, conserved motifs, and phylogenetic relationships of Dof genes. Gene expression analysis indicated that several Dof genes are involved in plant growth, development, and responses to environmental stresses. The results provide valuable insights into the functional roles of Dof transcription factors and establish a foundation for future studies on their regulatory mechanisms and applications in crop improvement.