Aims: The present study aimed to detect the frequency of v ancom y cin resistance and virulence genes’ profiles of multi-drug-resistant (MDR) enterococcal isolates from different sources and to in v estigate the sequence heterogeneity between the esp genes of MDR and vancomycin- resistant Enterococcus faecalis isolates from c hic ken and human sources. Methods and results: Con v entional phenotypic methods identified 91 isolates (60.7%) as Enterococcus species, and these isolates were retrie v ed from dairy (37/52), c hic ken (35/54), and human (19/44) origins. Enterococcal isolates were frequently resistant to rifampin (67%), and 38.5% of the isolates were MDR. Of the 22 v ancom y cin-resistant enterococci (VRE) detected isolates, 11 (50%), 9 (41%), 1 (4.5%), and 1 (4.5%) isolate were identified as E. faecium , E. faecalis , E. casseliflavus , and un-specified Enterococcus spp., respectively . Moreover , 22 (1 00%) and 19 (86.4%) isolates harbored vanA and vanB genes , respectively. Of note, gelE and asa1 genes were more prevalent among the tested isolates (95.5% each), and the multi-virulence criteria were detected among 68.2% of the examined isolates. The sequences of esp genes of E. faecalis from the c hic ken breast meat and human urine samples were 100% identical with other esp genes and pathogenicity islands on GeneBank, which is undesirable. Conclusion: Our findings require strict h y gienic measures during the processing of c hic kens and their b y -products to minimiz e the possibility of transmission of virulent enterococcal strains. Furthermore, the use of antimicrobials in poultry and animal production in de v eloping countries should be controlled to minimize the prevalence of MDR and VRE isolates in humans.