This study aimed to determine the frequency of multi-drug-resistant bacteria (MDR) found in patients with urinary tract infections (UTIs) and to analyze the antibiotic resistance patterns in these patients. Additionally, Selenium nanoparticles (Se-NPs) were synthesized using eco-friendly methods and conjugated with antibacterial proteins (7S and 11S globulins) from red kidney beans to enhance their antibacterial properties. The highest percentages of resistance were found for Amikacin (AK; 43 %), Cefuroxime (CXM; 42 %), Gatifloxacin (GAT; 38 %), Clindamycin (DA; 38 %), Ciprofloxacin (CIP; 36 %), Ceftriaxone (CRO; 35 %), Cefoperazone/Sulbactam (CES; 34 %), Nitrofurantoin (F; 31 %), Piperacillin (PRL; 26 %), and Amoxicillin/Clavulanic acid (AMC; 23 %). The 10 bacterial isolates resistant to more than or equal to 80 % of selected antibiotics were chosen and then identified. All ten isolates were confirmed to be Acinetobacter spp., Klebsiella pneumoniae, and E. coli. The syn thesis of Se-NPs was indicated by colour changes to ruby red and are preliminary substantiated by UV–Vis spectrum high peak absorption spectrum at 388 nm for Se-NPs, while the conjugated Se-NPs with 7S protein were 400 nm as well as Se-NPs with 11S protein were 420 nm. The TEM and DLS confirmed the formation of selenium nanoparticles of several sizes. Results showed that 11S globulin had the highest antibacterial activity of 7S globulin and isolated protein, recording the MIC at 125 μ proteins (protein isolates, 7S, and 11S) has MBC (125 bacteria.