This study investigates the effects of combining different nitrogen fertilizers with organic amendments and Azospirillum brasilense on wheat growth, yield, and nutrient uptake in saline calcareous soil, with a goal of enhancing crop productivity while reducing environmental impacts. The objective was to evaluate treatments involving ammonium sulfate (AS), urea, and sulfur-coated urea (SCU), mixed with organic amendments such as Moringa seed residues (MSR), biogas manure (BM), and vermicompost (Ver), along with Azospirillum brasilense (ASB). Among these treatments, SCU emerged as the most effective nitrogen source, especially when combined with Vermicompost and ASB. Key results include the following: the highest Chlorophyll a content of 1.96 mg/g f wt was observed in the SCU treatment with Ver and ASB; Likewise, the same treatment exhibited the highest values of Chlorophyll b, carotenoids, and proline; the tallest plant height (117.50 cm) was also achieved with this combination; and the highest straw and grain weights (2.63 g/plant and 2.22 g/plant, respectively) came from the same treatment. Additionally, this treatment yielded the highest protein content at 19.29%. In terms of nutrient uptake, SCU-based treatments consistently demonstrated the highest values. The SCU with Ver + ASB treatment recorded the highest nitrogen uptake in straw at 78.64 mg plant−1, phosphorus uptake at 12.86 mg plant−1, and potassium uptake at 61.66 mg plant−1. Similarly, this combination yielded the highest N, P, and K uptake in grains, with 68.05, 10.88, and 53.15 mg plant-1, respectively