The novel characteristics of nanofluids made them potentially important in the heat transfer mechanisms happening in industrial and medical proc esses, such as electronic devices, pharmacological techniques, hybrid com bustion engines, thermal management of cars, refrigerator, chiller, temperature of gases reduction, and so on. In comparison to base fluid, these procedures have a propensity to improve thermal conductivity and convective transference of heat. This distinctive aspect has recently gener ated interest in nanofluids through various fluid flow models. The issue at hand involves the peristaltic flow of magnetohydrodynamics Eyring–Powell nanofluids through a microchannel containing oxytactic bacteria and nano particles. For a thorough analysis of irreversibility optimization, the analysis of entropy is additionally taken into consideration. To comprehend the flow physics associated with bolus formation and the effect of reflux on the micro vessel, a 3-D computational fluid dynamic analysis is performed. The effects of bilateral chemical interaction with nonlinear mixed convec tion and Arrhenius activation energy are considered in the present analysis. The complexity of the system was achieved through a numerical approach based on the concepts of small Reynolds number and large wavelength. The resulting nonlinear coupled differential equation system is solved numerically using the built-in command in MATHEMATICA (Parametric ND Solve Function). To perform a thorough comparative examination for vari ous values of the considered sections of the channel, graphs have been used. Some of the investigation’s findings are the entropy profile has been improved for incremental values of Hartmann number and Brinkmann number whereas the axial velocity shows a downward trend for the high est values of the Hartmann number and the material fluid parameter.