Vibrio alginolyticus strain GH5 (OR865148.1) was isolated from the Red Sea, Hurghada, Egypt, and classified according to cultural attributes, biochemical properties, and the analysis of genetic relationships using 16S rRNA sequences. A substantial proportion of exopolysaccharides (EPS) in GH5-EPS contained a sulfate level of 19.8 %, uronic acid of 6.7 %, and N-acetylglucosamine of 16.07 %. The composition of monosaccharides in these fractions consists of arabinose, glucose, and galacturonic acid in a proportion of 2:1:2, respectively. GH5-EPS surface appeared as a needle-like shape by SEM. GH5-EPS enhancement of the RAW264.7 macrophage line ATTC number J774 cell proliferation via MTT assay for cell viability. GH5-EPS had a high immunomodulatory effect on releasing TNF-alfa and IL-10. Followed by its effect against cyclooxygenase (COX-2) and lipoxygenase (LOX). Antioxidant activity was evaluated for GPx4, GSS, and MDA with highly significant results for DPPH, ABTS, and iron chelating. AChE was inhibited by a mean of (30.25 ± 1.76 and 52.1 ± 2.77 μg/mL). GH5-EPS illustrated a good treatment effect for AD in a rat animal model, supporting biochemical studies, histopathology for some brain parts, and toxicity. Bioinformatic investigation for GH5 gene and translated protein structure and function provide insights for future drug-targeting of the EPS production pathway.