| Abstract: |
Drought is a major environmental stress, particularly in arid regions, where it severely limits faba bean productivity.
Foliar-applied ascorbic acid (AsA) and soil-applied humic acid (HA) signifcantly improved drought tolerance in three
faba bean cultivars by enhancing physiological performance and mitigating oxidative damage under moderate
(300 mm) and severe (200 mm) drought conditions. Drought stress signifcantly reduced chlorophyll content (up to
-57.5%), relative water content (RWC, -37.9%), and yield traits such as plant height (−9.6%) and seed yield (−20.8%),
while increasing oxidative stress markers like malondialdehyde (MDA, +192.8%) and hydrogen peroxide (H₂O₂,
+105.0%). AsA and HA alleviated these effects, improving chlorophyll retention (up to +33.7%), water status (+17.0%),
and reducing MDA(−19.1%) and electrolyte leakage (−11.5%). Enhanced accumulation of proline (+27.4%) and
soluble sugars (+18.0%) contributed to improved osmotic balance, while antioxidant enzyme activities (superoxide
dismutase, catalase, peroxidase, ascorbate peroxidase, and glutathione reductase) were also upregulated, particularly
with AsA. These treatments improved growth, yield traits, and water use efciency, especially under drought stress,
with Nubaria-5 showing the highest drought resilience. This cultivar exhibited superior pigment stability, antioxidant
activity, and yield preservation across stress conditions. Signifcant interactions among irrigation regime, biostimulant,
and cultivar highlighted the importance of genotype-specifc responses. Heatmap analysis confrmed the consistent
effectiveness of AsA, particularly under severe drought in Nubaria-5. Overall, AsA and HA function as effective
biostimulants for enhancing drought resilience in faba bean by improving photosynthetic efciency, water relations,
and antioxidative capacity, with AsA showing greater overall efcacy.
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