In terms of physical analysis; 82.9% of tested samples were nonconformed to temperature standard, while 100% were conformed to color and odor. Regarding chemical analysis; 54.3% and 53.3% of samples were nonconformed to alkalinity and combined chlorine, respectively. Assessment of microbial contamination indicated the prevalence of Pseudomonas aeruginosa as a main contaminator of tested pool water (57.1%) followed by total coliforms which ranked second among detected microorganisms (52.4%). Upon performing antimicrobial susceptibility testing of plant extracts and essential oils, cinnamon hot water extract and essential oil showed narrowest MIC ranges. Upon testing various combinations between different tested antimicrobial agents, combination of cinnamon hot water extract and clove essential oil was identified as a promising synergetic combination against resistant isolates. In field application of the most effective antimicrobial compounds resulted in that compatibility markedly decreased for physical standards and slightly for chemical standards. However, microbial pollution was totally eliminated after treatment, which was considered and discussed within the context of the study.