Clodinafop-propargyl (CF) is a widely used herbicide with high persistence in soil, raising concerns about its long-term environmental impact. Bacterial biodegradation provides a sustainable and eco-friendly strategy to mitigate CF contamination. This study evaluated the in-situ bioremediation potential of a six-member bacterial consortium (T3) by measuring CO₂ evolution as an indicator of microbial activity and monitoring CF residues in two soil types using microcosm systems. The consortium included Bacillus subtilis subsp. subtilis AZFS3 (LC599401.1), Bacillus pumilus AZFS5 (LC599402.1), Bacillus mojavensis AZFS15 (LC599403.1), Bacillus paramycoides AZFS18 (LC599406.1), Pseudomonas aeruginosa KZFS4 (LC599404.1), and Alcaligenes aquatilis KZFS11 (LC599405.1). T3 performance was compared to two individual strains representing Gram-positive and Gram-negative bacteria: B. subtilis subsp. subtilis AZFS3 (T1) and P. aeruginosa KZFS4 (T2). T3 achieved the highest degradation efficiency among all treatments, with CO₂ emissions nearing zero by day 20 (0.120 mg CO₂/100 g/DW. soil, equivalent to 0.63 % CF residue in sandy loam). CF residues were significantly reduced to 0.34 % (0.16 mg/kg sandy loam soil) and 6.38 % (2.99 mg/kg clay soil), underscoring the consortium’s strong bioremediation capacity, as determined by HPLC. Metabolite profiling, gene expression analysis, and pathway prediction further supported the consortium’s superiority over individual strains. The enzymes required for CF degradation were distributed across all six bacterial strains. The molecular characterization of the CF degradation pathway, using integrated bioinformatic and experimental approaches, confirmed its collective encoding by the individual members of the bacterial hexa-consortium. The Maleylacetate reductase (EC 1.3.1.32), crucial for the complete CF breakdown, was found exclusively in P. aeruginosa, suggesting a potential enzymatic bottleneck and a limiting factor. Future research should focus on enriching the consortium with additional strains expressing this enzyme to provide other alternatives capable of enhancing the degradation efficiency. The six-strain consortium offers a practical and environmentally sound solution for remediating CF-contaminated soils.