Background: Voice disorders, resulting from structural or functional changes in the larynx, affect about 30% of the population. Larynx structural or functional changes that affect the speaker's functional requirements can be the cause of these disorders. Aim: To assess voice after hyaluronidase injection in MAPLs of the vocal fold by voice handicap index. Patients and methods: This prospective cohort research involved 24 cases diagnosed as benign vocal fold lesions who underwent vocal fold injection of the hyaluronidase (HAase) enzyme in the phoniatric unit, ENT department, faculty of medicine, Zagazig University, from June 2022 until December 2023. Results: There was significant distinction among voice handicap index scores at different periods. There wasn't a statistically significant distinction among the two studied maneuvers with regarding the voice handicap index at baseline. There was a statistically significant distinction among the two maneuvers studied with regarding the voice handicap index at 1 month. There wasn't a statistically significant distinction among the two studied maneuvers with regarding the voice handicap index at 3 months. Conclusion: In minimally associated pathological lesions (MAPLs) (Reinke’s edema, polyps, & asymmetrical nodules), HAase enzyme injection is effective in improving voice, according to the results of the vocal handicap index.