Background: Viral conjunctivitis is an inflammation of the conjunctiva caused by viruses. Interleukin-4 (IL-4) and IL-4R have been reported to associate with the pathogenesis of conjunctivitis; however, the role of IL-4 and IL-4R genetic polymorphisms in conjunctivitis remains unknown. Aim: This study aimed to evaluate the relation between IL-4 gene polymorphisms (rs2243250) and IL-4 gene receptor (rs1805010) with infectious conjunctivitis. Methods: The study was conducted in 92 age-matched volunteers; 50 of viral conjunctivitis patients and 42 of healthy controls. The levels of both IL-4 and total IgE were measured using suitable methods. Genotyping of IL-4 (rs2243250) SNP was performed by Alleles Refractory Mutation Systems PCR (ARMS PCR) method, while the Genotyping of IL-4 receptor (rs1805010) SNP was performed by RFLP PCR method. Results: Our results showed that there is a significant difference in genotype and alleles distributions in IL-4 (rs2243250) SNP between viral conjunctivitis and control. However, no significant difference in genotypes and alleles distribution IL-4 gene receptor (rs1805010) SNP between viral conjunctivitis and control. More ever, there is a significant increase in serum IL-4 and total IgE in viral conjunctivitis than control. Conclusions: the analysis of our results indicates that IL-4 (rs2243250) SNP gene polymorphisms may be an indicator of increasing the incidence of viral conjunctivitis in the Egyptian population.