oods and cereals, resulting in serious threats to human and animal health. There is a lack of globally established approaches for ochratoxin A detoxification. Apigenin is a naturally occurring flavonoid that retains advantages linked to antioxidant, anti-inflammatory, and tumor suppressive effects. Investigating the possible ameliorative properties of apigenin against the hepatic toxicity induced by ochratoxin A in experimental rats. Four groups (8 rats each) were randomly assigned: control, apigenin (APG), ochratoxin A (OCA), and APG+OCA groups. Biochemical, molecular, histopathological, immunohistochemical and morphometric investigations were assessed. APG administration improved hepatic function by restoring liver enzymes, albumin level, and hepatic histopathology. APG restored the redox balance as evidenced by malondialdehyde alleviation and increased hepatic superoxide dismutase and glutathione peroxidase activities. Moreover, APG mitigated inflammation by restoring hepatic TNF-α and IL6 activities. In addition, APG moderated Nrf2/HO-1 and Wnt/β-Catenin/Cyclin D1 signaling pathways as indicated by Nrf2/HO-1 upregulation and Wnt/β-Catenin/Cyclin D1 downregulation. Furthermore, APG upregulated E-Cadherin and downregulated alpha fetoprotein gene expression. APG alleviated fibrosis and proliferation, as evidenced by Masson trichrome and immunostaining data of PCNA, respectively. This study provided novel findings highlighting the ameliorative potential of APG against OCA‐ induced hepatic oxidative damage, inflammation and hepatocellular proliferative signaling mediated via Nrf2/HO-1 and Wnt/β-Catenin/Cyclin D1 pathways.