Background: One of the most widely used dithiocarbamate fungicides around the world is Mancozeb (MZ). It is used in domestic, agricultural, and industrial applications which can increase its toxic effects. Lemongrass essential oil (LGEO) offers a broad scope of health benefits. This experiment aimed to investigate LGEO role in reducing renal toxicity induced by MZ in rats. Material and Methods: Seventy-five adult male albino rats were equally and randomly allotted into five groups (15 rats/group): The control groups (negative and vehicle), the Lemongrass group received LGEO 200 mg/kg, the MZ-treated group received 750 mg/kg MZ, and the combined group received LGEO with MZ as same doses mentioned before. The animals were treated orally for 8 weeks. Results: MZ Administration revealed a significant rise in serum creatinine, blood urea, and renal tissue malondialdehyde (MDA) levels, while superoxide dismutase (SOD) activities and glutathione content levels were significantly reduced in comparison to the control group. Also, the treated group with MZ revealed increased DNA damage and altered kidney histopathology, increased collagen deposit, with high expression of TNF-α, 8-OHdG, and p53 in renal tissue, as well as a high score of congestion, haemorrhage, infiltration, and necrosis. The rats treated with LGEO and MZ demonstrated partial improvement based on comet assay, renal oxidative stress parameters, and histopathological and immunohistochemical investigations. Conclusion: LGEO administration attenuates MZ induced renal injury via modulation of their oxidant, inflammatory, and apoptotic impacts. Recommendation: encourage use of safety measures as well as LGEO consumption among farmers to reduce MZ health consequences.