Grapevine (Vitis vinifera L. cv. Prime) cultivation in sandy soils of Egypt faces serious challenges due to infestations by root-knot nematodes (Meloidogyne incognita), which reduce yield and vine vigor. This study aimed to develop a sustainable management strategy using composted animal manures (CAMs) derived from poultry, cow, and sheep sources, along with plant growth-promoting rhizobacteria (PGPR), including Pseudomonas spp. and Serratia spp. Laboratory assays confirmed the strong nematicidal activity of both bacterial strains, demonstrating pronounced ovicidal and juvenile-lethal effects against M. incognita. While greenhouse and field trials demonstrated their efficacy in reducing nematode reproduction, root galling, and egg mass formation. Moreover, the combined application of CAMs and PGPR not only enhanced plant growth and grapevine productivity but also promoted beneficial free-living nematodes (FLNs), such as Rhabditis spp., and predatory nematodes (PNs), including Mononchus spp., contributing to improved soil health and ecological balance. These findings support the integration of organic amendments and rhizobacteria as an eco-friendly and effective alternative to synthetic nematicides in nematode-infested vineyards.