Chrysomya albiceps (Wiedemann 1819) is an important parasitic blow fly species known to cause myiasis in livestock and occasionally in humans. Its capacity to infest wounds and induce severe tissue damage makes it a significant species in both veterinary and public health fields. The accurate identification of fly species colonizing a corpse is crucial in forensic disciplines, serving as the initial step in estimating the minimum time since death. DNA barcoding is a powerful technique for identifying fly species besides traditional morphological identification. This study aimed to identify one of the most forensically important blow fly species, Chrysomya albiceps, collected from three regions in Egypt using the mitochondrial cytochrome oxidase subunit I (COX1) gene. Adult fly samples were collected from Menofia, Port-Said, and El-Sharkia governorates using meat-baited traps, morphologically identified, then DNA was extracted from C. albiceps specimens, and a region ~703 bp fragment of the COX1 gene was amplified, visualized, and finally sequenced. Sequence analysis revealed no significant intraspecific divergence in C. albiceps specimens captured from different geographical regions in Egypt. Accordingly, the neighbor-joining tree using the Tamura-Nei model illustrated reciprocal monophyly between species. Thus, these approaches serve as promising tools for molecular identification of this common forensically important blow fly species in Egypt which is considered as a very crucial step in forensic investigations