Improving sludge solubilization and biogas generation through thermal, alkaline, and hydrodynamic pre-treatment methods is considered one of the most important research points in the anaerobic digester field. Results revealed that the hybrid method is better than other methods as it achieved 0.64 (CODs/ energy consumed) ratio and the gas production rate ranged between (23.34 L/ kg COD.d) at RT 30 days. While thermo-alkaline mixed with AW (1: 1 by mass) is very effective in the digesters by reusing agricultural waste into useful biogas, reached at RT 30-day high gas rates (11.55 L/kg COD.d) and cumulative gas volume about (0.43 L/g of COD added), even though CO2 emissions initially limited the production. Compared to traditional digesters, pretreatment methods significantly decreased CO2 emissions early on. Additionally, heating agricultural waste with sludge above 100
C facilitated a reduction in sludge volume, as cellulose components in agricultural residues, particularly from sugarcane, absorb water from the sludge—this factor is crucial for minimizing the capital costs of drying beds. Overall, alkaline pretreatment improved biodegradability overall and shortened digester retention times.