Genomic Characterization of an Extensively Drug-Resistant Extra-Intestinal Pathogenic (ExPEC) Escherichia coli Clinical Isolate Co-Producing Two Carbapenemases and a 16S rRNA Methylase

Faculty Veterinary Medicine Year: 2022
Type of Publication: ZU Hosted Pages:
Authors:
Alaa El Din Mohamed Saad Meselhy
Journal: Antibiotics MDPI Volume:
Keywords : Genomic Characterization , , Extensively Drug-Resistant Extra-Intestinal Pathogenic    
Abstract:
An extensively drug-resistant Escherichia coli clinical isolate (N1606) belonging to Sequence Type 361 was recovered from the urine of a patient hospitalized in Switzerland. The strain showed resistance to virtually all _-lactams including the latest generation antibiotics cefiderocol and aztreonam–avibactam. Whole genome sequencing revealed that it possessed two carbapenemase encoding genes, namely blaNDM-5 and blaKPC-3, and a series of additional _-lactamase genes, including blaCTX-M-15 and blaSHV-11 encoding extended-spectrum _-lactamases (ESBLs), blaCMY-145 encoding an AmpC-type cephalosporinase, and blaOXA-1 encoding a narrow-spectrum class D ß-lactamase. Most of these resistance genes were located on plasmids (IncFII-FIA, IncX3, IncI, IncFII). That strain exhibited also a four amino-acid insertion in its penicillin-binding protein 3 (PBP3) sequence, namely corresponding to YRIN. Complete genome analysis revealed that this E. coli isolate carried virulence factors (sitA, gad, hra, terC, traT, and cia) and many other non-_-lactam resistance determinants including rmtB, tet(A), dfrA17 (two copies), aadA1, aadA5 (two copies), sul1 (two copies), qacE (two copies), qepA, mdf(A), catA1, erm(B), mph(A), and qnrS1, being susceptible only to tigecycline, colistin and fosfomycin. In conclusion, we described here the phenotypic and genome characteristics of an extensively drug-resistant (XDR) E. coli ST361 being recognized as an emerging clone worldwide.
    
     
 
       

Author Related Publications

  • Alaa El Din Mohamed Saad Meselhy, "Screening, isolation and characterization of large bacteriophages for use in biocontrol of a wide-range of pathogenic bacteria.", 2024 More
  • Alaa El Din Mohamed Saad Meselhy, "Zoonotic importance of campylobacteriosis at Sharkia province", 2024 More
  • Alaa El Din Mohamed Saad Meselhy, "In-vitro activity of newly-developed β-lactamase inhibitors avibactam, relebactam and vaborbactam in combination with anti-pseudomonal β-lactam antibiotics against AmpC-overproducing clinical Pseudomonas aeruginosa isolates", Springer Nature, 2024 More
  • Alaa El Din Mohamed Saad Meselhy, "Application of Bacteriophages for Biocontrol of Extensively Drug-Resistant Salmonella Serovars Isolated from Poultry Farms.", Assiut University-Faculty of Veterinary Medicine, 2023 More
  • Alaa El Din Mohamed Saad Meselhy, "Inactivation of Avian Influenza Viruses by Chemical Disinfectants and the Influence of Faecal Matter", Faculty of Veterinary Medicine-Alexandria Univessity, 2023 More

Department Related Publications

  • Heba Ahmed Abdallah Abdelmgeed, "Seafood a potential source of some zoonotic bacteria in Zagazig, Egypt, with the molecular detection of Listeria monocytogenes virulence genes", Istituto G. Caporale 2013, 2013 More
  • Abdallah Mohamed Amien Ali Marwad, "Prevalence of Non- O157 Shiga Toxin-Producing Escherichia coli and Enterotoxigenic Staphylococci in Ready-to-eat Meat Products, Handlers and Consumers in Cairo, Egypt إنتشار ميكروب الإيشيرشيا القولونىNon O157 المفرز لسم الشيجا وميكروب س", IDOSI Publications, 2014 More
  • Rasha Mostafa Mohamed Aboeleez, "The role of Atherina species in transmitting some bacterial diseases to human", http://www.jofamericanscience.org, 2014 More
  • Ahmed Magdy Ahmed Khalil, "Phylogenetic variations of highly pathogenic H5N6 avian influenza viruses isolated from wild birds in the Izumi plain, Japan, during the 2016-17 winter season.", ًWiley, 2019 More
  • Rasha Mostafa Mohamed Aboeleez, "Risk factors associated with Chlamydia psittaci infections in psittacine birds and bird handlers", Elsiever, 2019 More
Tweet