Heterakis gallinarum infection of turkeys is worldwide distributed causing severe economic losses. The current study was carried out to investigate the prevalence of Heterakis gallinarum in turkey in Egypt. For this purpose, a total of 90 intestines of domestic turkeys (Meleagris Gallopavo); 57 from Zagazig City and 33 from El-Salam Abattoir in Cairo Province were collected and examined during the period from October 2022 until January 2024. Furthermore, a descriptive comparison was carried out between each of the morphological and morphometric characterization in the adult and juvenile stages. The overall prevalence of the infection was 36.66%. The positivity reached 57.89% and 0% for the examined samples in Zagazig City and El-Salam Abattoir, respectively. The intensity of infection was 1-73 worms per infected bird with a mean of 22 worms. The comparative morphological and morphometric characterizations in adult and juvenile stages of Heterakis gallinarum were illustrated. Histopathological findings revealed a thickened mucosal layer with necrotic surface epithelium, intense leukocytic infiltration, and chronic nodular typhlitis. Sections from infected ceca showed a larval migratory tract within a deep submucosal layer surrounded by fibrotic capsule. Our study concluded the characteristic morphological differential features of Heterakis gallinarum male, female adult, and juvenile stages.