Background: a monkeypox (MPOX) outbreak occurred in May 2022. On June 3, 2022, the WhO Blueprint organized a consultation on MPOX research knowledge gaps and priority research questions because the engagement of health care providers (hcPs) in providing accurate information and the public's motivation to adapt protective behaviour were crucial. thus, we conducted this study to explore the knowledge issues, animal patterns, and interactions of hcPs in the context of MPOX and cOViD-19 during the MPOX outbreak. Methods: We conducted a cross-sectional web-based survey among 816 hcPs working in governmental health facilities from many countries, mainly syria, egypt, saudi arabia, and cameroon, in september 2022. Results: Four hundred and sixty (56.37%) were aged between 18 and less than 35 years old. about 34.44% were physicians, while only 37.25% worked on the frontlines with patients. 37.99% and 5.88% received vaccinations against chickenpox and MPOX, respectively. in the meantime, 55.39% had taken courses or training programmes regarding cOViD-19. Regarding knowledge-seeking behaviours (KsBs) about cOViD-19, 38.73% were through passive attention, while only 28.8% got their information through active search. Most of the participants (56.86%) had a moderate level of knowledge regarding cOViD-19. Only 8.82% had courses or training programmes regarding MPOX. Regarding KsB about MPOX, 50.86% were obtained through passive attention, while only 18.01% and 23.04% got their information through active and passive search, respectively. Most of the participants (57.60%) had a poor level of knowledge regarding MPOX. the regression analysis of the MPOX knowledge score revealed that individuals working on the frontlines with patients and those who had training programmes or courses were shown to have a higher score by 1.25 and 3.18 points, respectively. Conclusions: the studied hcPs had poorer knowledge about the MPOX virus than they did about the SARS-CoV-2 virus. training programmes and education courses had an impact on their knowledge.