Maximizing Power Point Tracking (MPPT) is an essential technique in photovoltaic (PV) systems that guarantees the highest potential conversion of sunlight energy under any irradiance changes. Efficient and reliable MPPT technique is a challenge faced by researchers due to factors such as fluctuations in irradiance and the presence of partial shading. This paper introduced a novel hybrid Equilibrium Slime Mould Optimization (ESMO) MPPT-based algorithm combining the advantages of two recent algorithms, Slime Mould Optimization (SMO) and Equilibrium Optimizer (EO). The ESMO algorithm is compared with highly efficient MPPT-based techniques such as SMO, EO, Particle Swarm Optimization (PSO), Grey Wolf Optimization (GWO), and Whale Optimization Algorithm (WOA), both under a Simulink environment and a real-time experimental laboratory setup using a Dspace1104 controller and PV emulator. The comparison focuses on performance under several irradiance cases, including instant irradiance change, partial shading, complex partial shading, and dynamic partial shading. The key advantage of ESMO is the fact that it has a single tunable parameter, which makes implementation much easier and, at the same time, reduces the computational resources that are required by the control system. Extensive testing proves the superiority of ESMO over all other techniques, the average efficiency of which is 99.98% under all conditions. Additionally, ESMO provides fast average tracking times of 244 ms under simulation experiments and 200 ms for real-time experiments. These results show that ESMO can be very important for future implementation in large-scale solar PV systems.