Despite advances in therapy of breast cancer, it still remains a significant health concern. During the past recent years, various research studies have encompassed that novel therapeutic approaches are urgently needed. Scorpion venom has emerged as a promising anti-cancer agent. This is achieved primarily via scorpion venom-derived peptides revealed high and selective affinity for ionic channels over-expressed in many cancer cells. In particular, This study refer to the anti-cancer properties of L. quinquestriatus venom against human breast cancer cells MDA-MB-231. Cell viability was assessed using MTT assay. Apoptosis induction was evaluated using Annexin V/PI staining kit. Wound healing assay was employed to analyze cell migration. The MTT assay results demonstrated that L. quinquestriatus venom decreased the cell viability and had a selectively cytotoxicity against cancer cells, owing to its possible anti-cancer activity. The Annexin V-PI staining revealed a substantial increase in the percentage of apoptotic cells upon L. quinquestriatus venom treatment, and the wound healing assay showed a marked reduction by (~70%) in the migration ability after L. quinquestriatus venom treatment in comparison with non-treated cells. Significant decrease in human breast cancer cell viability (~ 60%) was observed after 48 hrs. of treatment with L. quinquestriatus venom compared with non-treated cells (P < 0.0001). These present findings provide evidence that L. quinquestriatus venom possesses potent anti-cancer properties and may be useful in the development of breast cancer treatment strategies.