Introduction: Low frequency noise (LFN) is characterized by a sound with large pressure amplitude ≥90 decibel (dBL) and low frequency bands ≤500 Hertz (Hz). It has been accused in some cardiovascular and respiratory diseases. Objectives: to investigate the effect of LFN on the heart and lung of adult male albino rats, and the possible role of vitamin C supplement. Materials and methods: Fifty adult male albino rats were divided into two main groups; Control group (20 rats) which equally subdivided into two subgroups; Ia (received no treatment) and Ib: received vitamin C (70 mg/kg/d, by gavage). The experimental group (30 rats) which equally subdivided into two subgroups; Subgroup IIa: LFN-exposed and Subgroup IIb: LFN-exposed + vitamin C-treated subgroup. At the end of the experiment, blood samples were collected for blood analysis. Specimens from the myocardium of the left ventricle and lungs were processed for light and electron microscopic study and morphometrical and statistical analysis. Results: LFN had deleterious effects on the heart and lung including degenerative changes, cellular infiltration, fibrosis, and fatty infiltration. These changes were improved by vitamin C supplementation. Conclusion: Vitamin C had a protective effect on LFN induced myocardial and lung lesions.