Canine parvovirus (CPV) and canine distemper virus (CDV) are the common fetal disease of canine. Their control and prevention of these diseases include vaccination and high care should be done is important. in canine shelters throughout the world. Antibodies of these against both above viruses. The study helps to identify the goal was to confirm the specificity and sensitivity of ELISA and in comparison, compare it with CPV hemagglutination inhibition and CDV serum neutralization test. s using sera collected from dogs housed at animal shelters. The ELISA was used under both field and laboratory conditions and duplicate specimens were processed using an extra wash step. The accuracy of test kits in serum samples results showed that the sensitivity of CDV was 94.0%, using an optical density meter 88.1% with a specificity of 91.8%. For CPV, the sensitivity was 92.3% with 93.5% specificity. This test appears to be a good indicator for antibody detection against both virus.