The purpose of this investigation was to ascertain if orally administered Moringa oleifera (MO) extract had any protective effects on several biochemical markers in the kidney and liver in gentamicin (GNT)-induced hepato-renal toxicity in rats. Forty male albino rats were divided into four groups: the control group, the MO treated group, the GNT administered group, and the (MO+ GNT) group. The MO+ GNT group received GNT (100 mg/kg b.wt, i.p.) together with Moringa oleifera (400 mg/kg b.wt) for 20 consecutive days. Rats were put to death at the conclusion of the experiment, and blood samples were taken to measure serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total proteins, albumin, and globulin, as well as serum urea, creatinine, and uric acid. Catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), superoxide dismutase (SOD), total antioxidant capacity (TAC), 8-hydroxy 2’-deoxyguanosine (8OHdG), and malondialdehyde (MDA) were measured in isolated kidneys and liver. The liver and kidneys were divided into pieces for histology and a few immuno-histochemistry tests. Following administration of GNT, there was a significant decrease in the activities of the hepatic and renal CAT, GPX, GSH, SOD, and TAC while there was a significant increase in the levels of MDA, 8OHdG, serum AST, ALT, ALP, urea, creatinine, and uric acid. Treatment with MO significantly lessened the histopathological abrasions in the liver and kidney tissue brought on by GNT and restored the levels of renal and hepatic BAX and TNF. It also restored the evaluated criteria to normal values. According to the results, MO has a protective effect against GNT-induced hepato-nephrotoxicity in rats. This effect may be explained by the fact that MO prevents free radical generation and restores antioxidant activity, which reduces the negative effects of GNT.