Cryptosporidiosis is a major diarrheal disease with high mortality, primarily in young animals, and a zoonotic risk of infection. The molecular epidemiology patterns of Cryptosporidium isolates are still poorly understood in Egypt and other developing countries. The goal of this research was to better understand the genetic diversity of Cryptosporidium isolates in pre-weaned cattle calves in Egypt's Al-Sharkia governorate by examining the hemo-biochemical changes in infected calves