Structural cracks are the major cause of structural failure and significantly impact their strength and durability. Bacterial self-healing concrete as a sustainable structural Smart material offers potential advantages by autonomously repairing cracks, reducing repair costs, and eliminating human intervention. Most self-healing concrete bacteria have optimal growth temperatures around 25–37°C. Their metabolic activity can decline at higher temperatures, potentially affecting their ability to produce calcium carbonate and heal cracks. This study aimed to investigate the effectiveness of heat-resistant bacteria strains in self-healing concrete under high temperatures. The study examined the impact of temperature, bacteria type, and content on self-healing concrete properties at four different temperatures: room temperature, 60°C, 120°C, and 200°C. Several tests were performed to test the effectiveness of the bacteria strains …