Chicken manure is a valuable resource when properly managed, while mismanagement of manure often results in serious challenges and public health worries. The environmentally friendly management of chicken manure is critical for agricultural sustainability. One of the strategies for promoting sustainable management of chicken manure is the application of the UV technique. The present research was carried out to apply and evaluate the performance of a germicidal ultraviolet (UV-C) disinfection system as a sustainable technology for disinfecting chicken manure. The performance of a UV-C disinfection system was studied as a function of changes in UV-C intensity (980, 1470, and 1960 µW/cm2) and exposure time to UV-C (5, 10, 15, 30, 60, and 90 min). Performance evaluation of the UV-C system was carried out in terms of microbial count, disinfection efficiency, specific energy, and disinfection cost. Experimental results revealed that the optimal limits for reducing TBC, coliform, and E. coli count (1.5, 2.8, and 1.8 log CFU g−1), disinfection efficiency (96.58, 99.84, and 98.40%), specific energy (0.33, 2.93, and 0.16 kW.h/kg), and disinfection cost (0.024, 0.218, and 0.012 USD/kg) were achieved at a UV-C intensity of 1960 µW/cm2 and exposure times of 10, 90, and 5 minutes, respectively. According to this study, UV-C disinfection provides an eco-sustainable alternative to chemical composites for controlling microbiological contamination in chicken manure