The intestinal porcine epithelial cells–jejunum 2 (IPEC-J2) have been used as a successful in vitro cell line model to study porcine and human rotaviruses. This raised our concerns to investigate further the ability of two other members of the group A rotaviruses, bovine rotavirus (BRV) and simian rotavirus (SRV), to grow in these cells. Like in the homologous porcine rotavirus (PRV) infection, IPEC-J2 cells kept up their viability post-infection by both BRV and SRV. In addition, the percent of IPEC-J2 cells expressing the viral protein 6 (VP6) at 16 hours post-infection (hpi) by BRV and SRV (61.7±8.11% and 61.35±7.01%, respectively) was relatively close to that of PRV infection (67.3±1.59%). This percent decreased over time until it reached 37.8±2.36% for BRV and 42.63±5.98% for SRV compared to 61.31±14.07% for PRV 48 hpi. Both BRV and SRV could replicate successfully in IPEC-J2 cells. The pattern of the growth kinetic curve for the three rotaviruses in IPEC-J2 cells at 16, 32, and 48 hpi was relatively similar. The virus titers reached 7.2±1.0 log10 TCID50/mL in the PRV-infected cells and 4.5±0.47 and 4.7±0.8 log10 TCID50/mL in BRV- and SRV-infected cells, respectively, at 48 hpi. On 72 hpi, the virus titers reached 3.7±0.317 and 3.6±0.7 log10 plaque forming unit (PFU)/mL in BRV and SRV-infected cells, respectively, compared to 4.9±0.6 log10 PFU/mL in PRV-infected cells. Additionally, the viral RNA copies 72 hpi in both BVR and SVR infected cells were 5.01×105±0.55 and 3.98×105±1.2, respectively, compared to 7.94×105±1.04 in PRV infected cells. These results indicated that IPEC-J2 cells were productively infected by the heterologous BRV and SRV; and can serve as an intestinal cell culture model for studying both viruses.