Background: Abnormal uterine bleeding (AUB) is a common perimenopausal problem with numerous explanations and causes; thyroid disorders and hyperprolactinemia are part of these causes. This study aimed to find out the relationship between thyroid disorders and prolactin level in perimenopausal women with abnormal uterine bleeding. Methods: Cross sectional study was conducted on 63 patients aged from 40-55yr attending the outpatient clinic of Obstetrics and Gynecology Department Zagazig University hospital and Tallerak Central Hospital complaining from abnormal uterine bleeding. All women were subjected to examinations of the abdomen and Pelvic. The vagina was inspected with the use of a speculum. The bimanual examination was performed also. Laboratory investigation including Complete blood count, Liver function tests, Fasting blood glucose, Urine pregnancy test to exclude pregnancy. Specific investigations include thyroid gland hormones (TSH, free T3 and free T4) and serum prolactin. Results: There was negative non significant association between age, T3 (r=-0.14), TSH (r=-0.1) and Prolactin (r=-0.06). It also showed that there was positive significant correlation between abnormal uterine bleeding, hyperthyroidism (r=0.06) (p<0.05), hypothyroidism (r=0.1) (p<0.05), hyperprolactinemia (r=0.06) (p<0.05) and HMB (r=0.1) (p<0.05). This study showed that abnormal uterine bleeding in perimenopausal women is strongly associated with thyroid disorders and hyperprolactinemia. Conclusions: Abnormal uterine bleeding is strongly associated with thyroid-related disorders especially hypothyroidism at cut off value of TSH = 29.1 and also associated with hyperprolactinemia at cut off value of prolactin = 45.1.