Introduction. Plastic pollution is becoming a global reality. In recent years, numerous research papers have recognized the significance of public perception, knowledge, awareness, attitude, and behavior toward environmental problems. Objective. The aims of this study are to determine level and sources of Knowledge and behavior regarding safe plastic food containers (SPFCs) among female employees, and to evaluate the effectiveness of a health education program of SFPCs among them. Methods. An interventional study was conducted among female employees in Zagazig University, Sharkia Governorate, Egypt. A pre-post-test design was used in the evaluation of female employees’ knowledge and behavior regarding SPFCs. A health education program was conducted to improve their knowledge and behavior; then, a re-evaluation was done after three months. Results and conclusions. Most participants determined that easy availability, cheapness, and lack of rules\bans for plastic usage presented the highest scores for widespread plastics utilization (88.7%, 82.3%, 74.2%; respectively). Most participants (79.0%) gained their knowledge regarding the SFPCs from the social media mainly Facebook. After the implementation of the health education program, there was a significant improvement in the participants’ knowledge and behavior regarding SPFCs. Applying proper health education program for SPFCs gave promising outcome concerning knowledge and behavior.