Newcastle disease (ND) was first recognized more than nine decades ago and continues to be a problem for poultry producers besides being enzootic in many countries including Egypt. Diagnostic and ND virus/vaccine titration are core objects in virus evaluation. A rapid assay based on micro plate hemagglutination (HA) activity was applied to investigate its reliability as alternative method for virus titration. A comparative determination of EID50 /0.1ml was carried out via chicken embryo (CE) inoculation for 35 Newcastle disease virus (NDV) strains. They are previously identified by pathogenicity indices and revealed variable virulence (lentogenic-mesogenic and velogenic). The data for both methods were analyzed using SPSS version 25. A Wilcoxon signed-rank test showed that HA titer method did not elicit a statistically significant change in median of reading virus titer of samples with the median of standard embryonated chicken egg (ECE) method (Z = -0.197, p = 0.844). Spearman’s correlation coefficient (r= 0.42, p =0.01) showed a noteworthy moderate correlation between two methods. Heat map showed the differences between each pair of methods and the relationship between them. Bland-Altman plot revealed difference which fit normality distribution W=0.96, p=0.24. Accordingly, the use of HA activity assay for NDV/vaccine titration is a rapid easy and reliable, especially when needed for primary evaluation.