Background: Estimation of sex is an important item in identification of unidentified persons. The aim of the current work was to evaluate sacrum and coccyx gender differences using magnetic resonance imaging (MRI) in Egyptian population. Subjects and method: 230 Egyptian subjects (115 males and 115 females) were investigated with MRI over the sacrum and coccyx. MRI images of every person were used to get seven length estimations and two angle dimensions in the sacrum and coccyx. Results: Measured lengths including anterior sacral length (ASL(, posterior sacral length (PSL), anterior sacrococcygeal length)ASCL), posterior sacrococcygeal length (PSCL) and antero-posterior diameter of S1 vertebra corpus (APD) were higher in males. But there was no significant variation in maximum breadth of alae sacralis (MBA), maximum lumbo-sacral curve angle) MLSCA) and lumbo-sacral angle (LSA) between males and females. Univariate discriminant function analysis showed that ASL and PSL recorded 71.9% and 71.5% accuracy rates respectively. Stepwise analysis considered that (ASL and PSL) collectively produced 68.9% correct prediction rate. Conclusion: the results of the current work concluded that the sacrum and coccyx are valuable for gender differentiation, can be helpful for sex identification of skeletal remnants in the Egyptian when utilized with other methods as assessment of other readily accessible bones.