Rosa hybrida (cv. Red Dino) was grown at the experimental site of the Hort. Dept., Fac. Agric., Zagazig University, Egypt, during the two seasons of 2017 and 2018. In order to estimate the effect of different amino acid types (glutamine, methionine, phenylalanine and proline), soilless culture methods (nutrient film technique and solid substrate culture) and their combination treatments on growth and flowering of rose plant. These experiments were designed as a split-plot in complete randomized block design with three replicates. The results showed that in most cases phenylalanine acid gave the highest values of growth parameters (plant height, number and weight of leaves, leaf length and width as well as root length) compared to the other amino acid types under study. The same trend was obtained concerning flowering parameters (flowers number, weight and diameter per plant, flower stalk length as well as vase life of cut flowers) during both seasons. The solid substrate culture (SSC) in most cases gave correspondingly higher values of above mentioned parameters (except that of flowering date and flowers vase life) than the nutrient film technique (NFT). Total chlorophyll content (SPAD) was significantly increased with SSC compared to NFT. The best combination treatment, from the productive point of view, was treating plants with phenylalanine acid at 200 ppm and culturing in solid substrate soilless culture (mixture of granulated sand and peat).