Background: In forensic practice, examining wounds is one of the most important tasks of a forensic pathologist. It is necessary to estimate how much time has passed since each wound was sustained, which is known as wound age estimation. The age of a wound refers to the length of time a person continues to live following an injury. It helps with criminal investigations by verifying the occurrence and development of violent incidents and pinpointing possible criminal suspects. A diversity of methods for estimating wound age have been established, and the recent developments in forensic methods allow for the simultaneous examination of wounds at both the cellular and molecular levels, in addition to assessing different markers. Aim: The current work provides a review of the different methods applied for wound age estimation in the field of forensics. Conclusion: Researchers have pointed out many techniques for estimating the age of a wound including regular histopathological analysis, immunohistochemical staining, biochemical examination and reverse transcription polymerase chain reaction (RT-PCR). Immunohistochemistry is the preferred technique in forensic pathology due to its simplicity and dependability. Advancements in molecular biology have enabled the utilization of mRNA for forensic purposes. The mRNA levels of wound healing factors and inflammatory markers can accurately determine the age of wounds in their early stages. In the future, there is a need to establish a system for estimating the age of wounds at both the molecular and protein levels.