ABSTRACT Background: Obesity can modulate gastric myoelectric activity (GMA); however, the relationship of GMA with nutrient intakes and substrate utilization in adults with obesity is lacking. Aim of the Study: We examined the association of dietary intakes, energy expenditure, and substrate utilization with the GMA. Material and methods: Participants (n = 115, 18–60 y) were divided into healthy weight (HW, n = 24), overweight (OW, n = 29), obese (OB, n = 41), and morbidly obese (MO, n = 21). Two-day multi-pass 24-hour recalls were conducted. The GMA was measured by multichannel electrogastrography (EGG) with water-load (WL) testing. Resting metabolic rate (RMR) and percentages of substrate utilization were measured by indirect calorimetry. Results: In the HW, protein intake was directly correlated with average dominant frequency (ADF) and with WL volume, while in obese participants and the MO subgroup, WL volume correlated with carbohydrate intake. In participants with obesity, ADF was positively correlated with fiber intake. In participants with obesity and the OB subgroup, RMR was positively correlated with waterload volume (r = 0.39 and 0.37, p < 0.05). The ADF showed negative correlations with percent of fat utilization and positive correlations with percent of CHO utilization in non-obese groups. However, protein utilization showed inverse correlation in all obese groups. Conclusion: In conclusion, these distinctive associations suggest that certain dietary compositions and dieting regimens impact GMA patterns. Keywords: Gastric myoelectrical activity; substrate utilization; energy expenditure; dietary intake; obesity