In this study, mixed ligand complexes derived from levofloxacin as primary ligand and glycine as secondary ligand have been prepared and characterized by conventional techniques including elemental analyses, infrared, electronic spectra, 1H NMR, thermal analyses and XRD. The infrared spectral data show that the chelation behavior of the ligands toward transition metal ions is through pyridone, carboxylic group of levofloxacin and nitrogen atom of amine group and one oxygen atom of carboxylic of glycine. Thermal analyses show that the complexes lose water molecules of hydration initially and subsequently expel anionic part and organic ligands in continuous steps leaving metal or metal oxide as a final product. The synthesis and characterization of five mixed ligand complexes with Zn(II), Zr(IV),La(III), Th(IV) and U(VI) have been synthesized with physicochemical and spectroscopic methods as well as thermal analyses. In all complexes, Levo acts as bidentate ligand bound to metal ion through pyridone oxygen and one carboxylate oxygen whereas Gly coordinate through nitrogen atom of amine group and one oxygen atom of carboxylic group.