The up-flow anaerobic sludge blanket UASB concept relies on high levels of biomass retention through the formation of granules sludge. In recent year, numerous designs and/or configurations of these reactors have been developed to optimize the anaerobic treatment of wastewater. The system can afford treatment for high strength wastewater at low energy requirement, low operational cost and low sludge production as well. Among the limitation of the UASB system are their limited treatment efficiency and the long start up phase. The main objectives of the present search is to study the effect of seeding Effective Microorganism (EM1) upon the UASB reactor treatment efficiency and establishes the optimum seeding dose and examine the seeding frequency. A pilot scale model was built simulating the UASB reactors and seeded with EM1 for four runs. In the first three runs the EM1 was seeded to the reactor on a daily base with volumetric rate of 0.21 %, 0.42 % and 0.85 %. In the fourth run the EM1 was seeded once with volumetric rate of 10.6 %. Based on the experimental program, it was found that, there was a remarkable improvement in COD and SS removal ratios by seeding with EM1. The best COD removal efficiency achieved was 99% for EM1 seeded at volumetric rate of 0.85% of the reactor volume. The study showed that the daily seed of the EM1 is recommended than the batch feeding regime.