Statement of The Problem: Since the fluoride releases from materials with the property of releasing fluoride is decreasing gradually, it seems that probably the material rechargeability is more important than its long-term fluoride release. Objective: This study aimed to asses and compare the fluoride releasing potential and recharging capacity of different bioactive restorative materials in-vitro. Materials and Methods: The sample consisted of 50 freshly extracted premolar teeth. Standardized buccal class V cavities were prepared. Samples were randomly divided into 5 main groups (n = 10 /group), representing materials used; Conventional glass ionomer cement (CGIC) ; G C Fuji IX G P, resin modified glass ionomer restoration (RMGI); Fuji II LC, Compomer; Dyract XP, Giomer; Beautiful II and one enhanced RMGI; ACTIVA Bioactive-Restorative. Each material was evaluated for its fluoride releasing potential and recharging capacity after topical application of fluoride varnish at 1st, 3th,7th, 14th, 21th and 28th days. Results: There was a statistically significant difference between each tested material at all time intervals before and after recharging with topical fluoride varnish where (p ≤ 0.001). The highest mean value of fluoride releasing potential and recharging capacity was in (Day-1), and the least mean value was in (Day-28). There was a statistically significant difference between the tested materials at each time interval regarding fluoride release before and after recharge.; CGIC (GC Fuji IX GP) showed the highest mean values of fluoride releasing potential and recharging capacity at each time interval (1st, 3th,7th, 14th , 21th and 28th days), while the lowest mean values were observed in compomer (Dyract XP ) at each time interval (1st, 3th,7th, 14th , 21th and 28th days). Conclusion: The CGIC was considered to be the material with the highest fluoride releasing potential and recharging capacity, while compomer showed the lowest fluoride releasing potential and recharging capacity at each time interval. It was observed that the enhanced RMGI (ACTIVIA Bioactive-Restorative) showed a lower fluoride releasing potential and fluoride recharging capacity than those of CGIC (GC Fuji IX GP), higher values than those of both Compomers and RMGI and comparable to those of Giomer at each time interval.