Two field experiments were carried out at one of the farm of EL Zawiah Zone , Libya during 2006 / 2007 and 2007/2008 season to study the response of barley plants cv California mariot to cheep manure ( 0, 50, 75 and 100 m3/ hectare )and nitrogen fertilizer such as, urea (46 % N ) (100 ,150 and 200 kg N / hectare). A split-plot design with three replicates was used in both growing seasons. The results revealed that, application of organic manure at the rate of 75m3/hectare gave highest values on vegetative characters(plant height, number of tillers/m3,number of spikes/m3,spike length and flag leaf blade area) and total chlorophyll, as well as yield and its components(1000-grain weight, grain yield Ardab/hectare and straw yield). By increasing the nitrogen fertilization level up to 200 kg N/hectare gave too the highest values of the characters above mentioned. Regarding the interaction effect, the treatment of 75m3 cheep manure/hectare and 200kg N/hectare, was recorded the highest values of most studied characters in this respect.