Several plant-parasitic nematodes have been associated with banana (Musa cavendishii) and some of the most economically important ones are Radopholus similis and Meloidogyne spp. the purpose of this study is mainly to clarify the pathogenic effects of both nematodes (Radopholus similis and Meloidogyne incognita, alone or in combination), as a histological modification in root tissues under natural conditions of banana cultivation. R. similis was observed in the cortical parenchyma as a feeding site, and for the first time, the feeding site extended to the vascular cylinder. In roots infested with M. incognita only, the laceration of the feeding site in the inner cortical tissue and spread to the vascular cylinder were selected as the initial feeding site, as shown. Multinucleate cells, giant cells, and thickening of cell walls were also associated with this penetration. The cytoplasmic globules as phenolic and lignified structures were shown to be associated with the feeding sites of two species. In combination with two species, the effects of M. incognita were noted more than those of R. similis. Where showed R. similis was protruding out or was inside the feeding site of M. incognita. In addition, R. similis was deepened within the root more than usual, with a lower number of phenolic and lignified cells. The obtained results can form the basis for understanding of the nature of nematode parasitism and be used as a basis for the establishment of field experiments that allow the creation of sustainable management strategies to suppress nematodes in infested fields.