The medical importance of dust mites as a predisposing factor for allergy in human has gained the attention of researchers over last two decades. These organisms spread in any site where dust can accumulate causing different types of allergies in a remarkable number of populations all over the world. This study was designed to investigate the effect of housing criteria and seasonal climatic changes on mite populations in dust samples isolated from different sites in rural and urban houses in Sharkia Governorate, Egypt. 33 houses representing different geographical areas in Sharkia Governorate were included and investigated for the presence of domestic mites. 66 dust samples were taken from floors of bed rooms, living rooms, kitchen and animal/poultry/bird breeding sites and examined for detection of mites using floatation method. 26 (78.8%) of houses showed positive results for mites. A statistically significant relationship was recorded between residence in rural areas, presence of birds’ nest or poultry in home, old furniture and dusty floor of houses, damp homes and large family size and the presence of mites in dust samples. 26(50%) of mite positive samples were isolated from bed rooms. Spring and winter had the most positive influence on mite population. 52 (78.8%) of samples collected were found positive for dust mites. Mean mite count per gram dust recorded high in spring samples (13 mite/gm dust) followed by winter and autumn samples (12 and 10 mite/gm dust respectively). Summer samples recorded lowest mite number in dust (6 mite/gm dust). Density of mites in dust samples recorded non-significant relationship with site of collection and seasonal variation. The result of our study confirm that housing criteria greatly determine mite occurrence in indoor environment.