Journal: |
Iranian Journal of Basic Medical Sciences
Mashhad University of Medical Sciences
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Volume: |
26
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Abstract: |
Objective(s): This study aimed to investigate the possible effects of fetuin-A on an adenine-induced
chronic kidney disease (CKD) model in male rats.
Materials and Methods: Rats were divided into three groups: group A included rats fed a normal diet;
group B included rats fed a normal diet with 220 mg/kg adenine daily for 21 days; group C included
rats fed a normal diet with 220 mg/kg adenine daily for 21 days and intraperitoneally administered with
5 mg\kg fetuin-A every other day for 2 weeks. Serum samples were assayed for serum creatinine, urea,
sodium, potassium, calcium, phosphorus, tumor necrosis factor (TNF), interleukin-6 (IL-6), and estimated
glomerular filtration rate (eGFR), and immunohistochemical staining was performed.
Results: Group B showed a significant increase in serum creatinine, urea, phosphorus, potassium,
TNF, and IL-6 and a significant decrease in serum sodium, calcium, and eGFR compared with group
A. Regarding immunohistochemistry, group B showed increased apoptosis. In group C, fetuin-A
reduced the urea, creatinine, and phosphorus levels, and in group C, fetuin-A decreased inflammation
and apoptosis by reduction of caspase-3 staining.
Conclusion: Fetuin-A improved kidney function in CKD due to its anti-inflammatory and anti-fibrotic
role.
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